Gram Stain - negative, the cell wall of the bacteria will...

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Aaron Miller BLD 213 A40386319 Gram Stain Tutor 1) Step 1: You want to Flood the slide with crystal violet reagent for about one minute and then rinse with water Step 2: Then Flood the slide with Gram’s iodine reagent for one minute and rinse with water. Step 3: Now you want to decolorize the slide by gently rinsing the slide with acetone- alcohol for ten seconds and then rinse with water. Step 4: Flood the with safranin reagent for one minute, rinse with water, and sit to dry. Dark Purple – The gram stain is positive for bacteria and yeast. Pink – The gram stain is negative for bacteria and host cells 2) There are characteristics for an organism that will determine whether the gram stain with be positive or negative. This includes the chemical and physical properties that lies within the bacteria’s cell wall. The color also depends on the permeability of the cell wall. If the gram stain comes out
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Unformatted text preview: negative, the cell wall of the bacteria will have more lipopolysaccrides in the outer membrane. Positive gram stain bacteria have a thick-mesh like cell wall, while negative gram bacteria will have a thin cell wall with phospholipid bilayer membrane. The negative gram stain bacteria will also Aaron Miller BLD 213 A40386319 Gram Stain Tutor be able to release the dye after it has entered the cell, because of its thin cell wall. 3) Gram positive for bacteria a. cocci- spherical bacterium Diplococcus (Pairs of cocci), Chain (chains of variable length), Tetrad (4 cocci), Cluster ( groups of cocci) b. Rod- rectangular shaped bacteria Long -wide (Blunt ends), Long narrow (blunt ends that may form chains of variable length), Coccobacillus (very short narrow rods). Branched (long and narrow, may have beaded staining pattern)....
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Gram Stain - negative, the cell wall of the bacteria will...

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