ex10 - Analysis 1.) The detergent sodium dodecyl (SDS)...

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Analysis 1.) The detergent sodium dodecyl (SDS) breaks the phospholipid bilayer, making it soluble and causing the cell to lyse. The function of the alkalinity is to denature the structural proteins incorporated with the cell by giving them less affinity for each other. This allows for the isolation of the plasmid DNA, and high pH also causes DNA to become denatured. Chromosomal DNA also becomes denatured, and is separated from plasmid DNA because chromosomal DNA is precipitated in the presence of SDS and high salt concentration, while smaller molecules of DNA, like plasmids, are not. 2.) If plasmid DNA is brought to a neutral pH, it tends to form a native helix conformation, even if the change is abrupt. Chromosomal DNA will also reform its native helix, but only if it is allowed to return to physiological conditions slowly and gradually. If this is fast and abrupt, irregular bonding will occur. 3.) Because nucleotides are insoluble in ethanol.
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This note was uploaded on 11/30/2008 for the course BIO 205L taught by Professor Hanson during the Fall '07 term at University of Texas at Austin.

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ex10 - Analysis 1.) The detergent sodium dodecyl (SDS)...

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