FAME - FAME, Analysis of Fats and Oils By: Shawn Thomas...

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FAME, Analysis of Fats and Oils By: Shawn Thomas Chem 243-B-009 Dates Performed: October 15, 2008 Date Submitted: October 22, 2008 Instructor Chen Abstract: In this experiment, we performed a saponification reaction with an Unnkown oil or fat (Triglyceride) in which they were carboxylated in acidic conditions and the acid was esterfied by a Methanol/ Aluminum Trichloride mixture. An extraction with the aid of a separatory funnel allowed us to isolate the unknown triglyceride which could then be characterized by Gas Chromatographic analysis. From the GC chromatogram, I determined the retention time for the Unknown to be ~8 minutes and a total fatty acid composition % to be approximately 7.5% which correlated strongly with the fat Lard . Introduction:
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In this experiment, we will be given an unknown commercially available fat or oil and we will then determine the fatty acid composition by Gas Chromatographic Analysis of the resulting eluted esters. Fatty acids almost all possess an even number of carbon atoms with sixteen and eighteen being the most frequent. FAME is an example of a saponification reaction in which hydrolysis of the ester under basic conditions produces the alcohol and the salt of a carboxylic acid. The production of soap is an example of a saponification. The final product of the reaction of this experiment will yield a transesterfied product which can be
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This note was uploaded on 12/04/2008 for the course BIO 181 taught by Professor Jorstad during the Spring '07 term at University of Arizona- Tucson.

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FAME - FAME, Analysis of Fats and Oils By: Shawn Thomas...

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