Bio Lab practical review pt. 1

Bio Lab practical review pt. 1 - BIO 205 Review Exam...

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BIO 205 Review Exam covers (Lots of stuff) Making solutions – Microscopy Population kinetics – Chromatography Urchin and root embryonic development Protein Extraction Protein electrophoresis & Western Blotting Plasmid Ligation and Bacterial Transformation Isolation of Plasmid DNA Analysis of Plasmid DNA Bioinformatics and Protein Domains Bioinformatics and Phylogenies And more… Sample Past exams Laboratory Exercises --> Practical Exam • Remember these are past exams So some Remember these are . So, some things, e.g., the amylase enzyme lab, weren’t covered this semester. Stations 1. Specific equipment • 2. Microscopy 3. Solution preparation: measurements, Solution preparation measurements, dilutions, pipetting, pH meter, spectrophotometer, balance, etc. • 4. Specific exercise details 5. Graphing & data interpretation 6. Calculations and equations Remember Be on time for the section you signed up for. Please no talking in the hallways Please, no talking in the hallways during this week. If you wish to review with someone, do it else where. Solutions Prepare 75 mL of a 1.5M sucrose stock solution: FW M conc. volume 342.3 g mol × 1.50 mol 1000 mL soln. × 75 mL soln. = 38.5 g sucrose in 75 mL of solution Solutions Prepare 25 mL of a 1.25 M dilution from the stock solution. C 1 V 1 =C 2 V 2 (1.50 M sucrose stock) v 1 = (1.25 M sucrose stock)(25 mL) V 1 = 20.8 m stock sucrose solution to make 25 mL of solution
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Serial dilution of salivary amylase Parallel dilution {C 1 V 1 =C 2 V 2 } V 1 stock C 1 C 2 V 2 Where? In vitro means within a test tube, or just outside a living organism (a cell). An example is In Vitro Fertilization. In vivo (Latin for (with)in the living) means within a living organism (a cell). For example, biochemical reactions can take place in vivo. In situ (a term used in biology) means to examine the phenomenon exactly in place where it occurs (without removing it in some special medium etc.). Usually means something intermediate between in vivo and in vitro. MICROSCOPY Bright field Phase contrast • Dark field Dark field • Flouresence Some microscope stuff… Magnification (ocular X objective) Resolution: distance (nm) between two points Abbe’s equation: d=0.5 λ /NA obj Kohler resolution Depth of field (low power vs. high power) Field of vision (low power vs. high power) Working distance (low power vs. high power) Ocular reticule Stage micrometer (2 mm ruler)
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Zone of cell differentiation Root cap Zone of division (meristem) Zone of cell elongation X
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X Y Z Pluteus Larvae Hemocytometer Counting sperm Dilution Factor (df)
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