Fall 05' Lab Practical

Fall 05' Lab Practical - Bio205L Fall 2005 Name UTEID...

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Bio205L Fall 2005 Name: UTEID: Page 1 of 6 Exam ptest059_12 STATION 1 Specific Equipment (10pts total; 2pts each) 1.1 The most practical non-electronic instrument to quickly but accurately weigh two liquid samples of similar volume (one a cellular extract, the other containing water to be used as a counter balance during centrifugation) is a(n)… a. analytical balance b. double-pan balance c. double-beam balance d. top-loading balance e. triple-beam balance 1.2 The best technique used to separate various proteins in an aqueous sample based on their chemical properties is called… a. affinity chromatography b. agarose gel electrophoresis c. ammonium sulfate precipitation d. electroblotting e. organic extraction 1.3 The best technique used to separate intact functional chloroplasts from other cell components and debris is called… a. affinity chromatography b. ammonium sulfate precipitation c. differential centrifugation d. tissue homogenization e. SDS-polyacrylamide gel electrophoresis 1.4 The equipment used in the final grinding step of the procedure to isolate liver proteins… a. blender b. homogenizer with a ground-glass pestle c. electrotransfer unit d. membrane dialysis e. mortar and pestle 1.5 The laboratory device used to estimate the number of cells in a solution of known volume based on its optical density is a(n) … a. hemacytometer b. media agar plate c. spectrophotometer d. stage micrometer e. UV transilluminator
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Bio205L Fall 2005 Name: UTEID: Page 2 of 6 Exam ptest059_12 *STATION 2 Solution Preparation, Measurement, Dilutions, Pipetting, etc . (10pts total) ********************************************************************* At this station, you have all the materials necessary to prepare a solution as directed, including a spectrophotometer, pH meter, and top-loading balance. If needed, the pipet wash jar is also located at this station (behind the computer cart) for proper disposal of used glass calibrated pipets. NOTE: no calculations are necessary; you are simply to perform the tasks as specified. Read the entire set of instructions before starting. Next, examine all the items available at this station. Select the BEST (most accurate/effective) devices/instruments to properly prepare the solution as directed and perform the specified tasks with good laboratory technique.
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Fall 05' Lab Practical - Bio205L Fall 2005 Name UTEID...

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