Fall 06' Lab Practical - Bio205L Fall 2006 Name: UTEID:...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Bio205L Fall 2006 Name: UTEID: Page 1 of 6 Exam p069test_04 STATION 1 Specific Equipment (10pts total; 2pts each) 1.1 The most accurate device to measure a 185 mL volume of liquid is a(n): a. 200-mL graduated Erlenmeyer flask b. 200-mL graduated cylinder c. P200 automatic pipetter d. 200-mL beaker e. top-loading balance 1.2 The best technique used to separate various proteins in an aqueous sample based on their chemical properties is called… a. affinity chromatography b. agarose gel electrophoresis c. electroblotting d. organic extraction e. spectrophotometric analysis 1.3 The best technique used to separate and concentrate DNA from other cell components is called: a. ethanol precipitation b. polyacrylamide gel electrophoresis c. thin layer chromatography d. ammonium sulfate precipitation e. tissue homogenization 1.4 The equipment used in the initial grinding step of the procedure to isolate liver proteins… a. blender b. electrotransfer unit c. homogenizer with a Teflon pestle d. homogenizer with a ground-glass pestle e. mortar and pestle 1.5 The equipment used to visualize DNA fragments from a restriction digest… a. hemacytometer b. media agar plate c. spectrophotometer d. stage micrometer e. UV transilluminator
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Bio205L Fall 2006 Name: UTEID: Page 2 of 6 Exam p069test_04 *STATION 2 Solution Preparation, Measurement, Dilutions, Pipetting, etc . (10pts total) ********************************************************************* At this station, you have all the materials necessary to prepare a solution as directed, including a spectrophotometer, pH meter, and top-loading balance. If needed, the pipet wash jar is also located at this station (beneath the table) for proper disposal of used glass calibrated pipets. NOTE: no calculations are necessary; you are simply to perform the tasks as specified. Read the entire set of instructions written below, examine all the items available at this station, and plan your steps in advance of performing any task. Be sure to select the BEST (most accurate / effective) item of all devices/instruments to properly prepare the solution as directed. Perform the specified tasks with good laboratory technique. Prepare a working solution from the “Chlorophyll” stock solution and obtain an absorbance reading at a fixed λ of 650nm , according to the following procedure. a.
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 6

Fall 06' Lab Practical - Bio205L Fall 2006 Name: UTEID:...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online