Fall 06' #2 lab practical

Fall 06' #2 lab practical - Bio205L Fall 2006 Name: UTEID:...

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Bio205L Fall 2006 Name: UTEID: Page 1 of 6 Exam p069test_12 STATION 1 Specific Equipment (10pts total; 2pts each) 1.1 The most practical instrument to quickly but accurately balance two 15 mL samples immediately prior to centrifugation is: a. analytical centrifuge b. double-pan balance c. double-beam balance d. clinical centrifuge e. triple-beam balance 1.2 The most effective instrument to pellet 15 μ L of ethanol precipitated DNA is a(n)… a. miracloth filtration b. clinical centrifuge c. microcentrifuge d. membrane dialysis e. None of the above 1.3 The best technique used to separate differently charged proteins in an aqueous sample is called: a. ethanol precipitation b. membrane dialysis c. differential centrifugation d. ion exchange chromatography e. electroblotting 1.4 The best device/technique used to collect plant cell debris and obtain brei is … a. chilled blender b. microcentrifuge c. homogenizer with a Teflon pestle d. cheesecloth filtration e. mortar and pestle 1.5 Consider a fluorescence microscope that uses yellow light (540 - 590 nm) to excite a fluorescent pigment in a cell. Then without knowing the pigment, which one of the following colors (wavelengths) of light would be most likely to be emitted by the fluorescing pigment? a. Ultraviolet light (395 nm) b. Blue light (440 nm) c. Green light (520 nm) d. Orange light (630 nm) e. None of the above
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Bio205L Fall 2006 Name: UTEID: Page 2 of 6 Exam p069test_12 *STATION 2 Solution Preparation, Measurement, Dilutions, Pipetting, etc . (10pts total) ********************************************************************* At this station, you have all the materials necessary to prepare a solution as directed, including a spectrophotometer, pH meter, and top-loading balance. If needed, the pipet wash jar is also located at this station (beneath the table) for proper disposal of used glass calibrated pipets. NOTE: no calculations are necessary; you are simply to perform the tasks as specified. Read the entire set of instructions written below, examine all the items available at this station, and plan your steps in advance of performing any task. Be sure to select the BEST (most accurate / effective) item of all devices/instruments to properly prepare the solution as directed. Perform the specified tasks with good laboratory technique. Prepare a Bradford Assay sample and measure its absorbance , according to the following procedure. a.
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This note was uploaded on 12/08/2008 for the course BIO 205L taught by Professor Hanson during the Fall '07 term at University of Texas at Austin.

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Fall 06' #2 lab practical - Bio205L Fall 2006 Name: UTEID:...

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