Discussion Sheet 9 Key - GENETICS (BIO 325) Spring 2008...

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GENETICS (BIO 325) Spring 2008 Discussion sheet 9 key _____________________________________________________________________________ _ 1. Bam H1 and Bgl II; 5'-GATC-3' 2. Some cohesive ends can form perfect Watson-Crick pairs, and therefore can be ligated; others cannot. Ligations (1) and (2), each involving two ends created by a single restriction enzyme ( Bam H1 and Xba I, respectively), regenerate a restriction site for the corresponding enzyme. Ligation (4) is successful because the cohesive ends are compatible, but ligation creates a "hybrid" hexanucleotide sequence that is not a restriction site for either Xba I or Nhe I. Ligations (3) and (5) cannot occur. 3. (a) 5'-AT-3' 5'-CG AT-3' Overhangs are underlined. 3'-TAGC -5' TA-5' (b) 4 (c) Cla I: cannot be determined Taq I: yes 4. 5. (a) Bam H1: one 4 kb; Bgl I: three 0.3, 1.5, 2.2 kb; Eco RI: one 4 kb; Pst I: one 4 kb (b) Two 0.7, 3.3 kb
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Discussion Sheet 9 Key - GENETICS (BIO 325) Spring 2008...

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