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Unformatted text preview: 4 Steps for PCR 1.) Collect DNA sample: cells, saliva, hair, blood, semen 2.) Isolate: separate DNA from other cell components to make it pure 3.) PCR: cut DNA into segments, then make copies (from 1 strand to 1 billion) 4.) Gel electrophoresis to determine pattern Epigenome-markers + switches along DNA that turn the expression of genes on and off-sensitive to cues from environment-a toxin or diet can affect the epigenome-this influence can be passed onto future generations (up to 4)-possible factors for cancer, depression, schizophrenia, dementia, Alzheimer, autism DNA Fingerprinting h unique array of DNA sequences for each person--smaller tandem repeats migrate faster & farther through viscous gel than longer ones h staining to highlight pattern -thus diet, drugs, or motherhs care (via release of serotonin) can influence offspring Sheet1 Page 2 HOX): linked gene complexes that develop a body plan...
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This note was uploaded on 12/11/2008 for the course BIO 213 taught by Professor Lee during the Spring '08 term at Cal Poly.
- Spring '08