nov11 - Outline of Chapter 12 To Clone a DNA Library?...

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Unformatted text preview: Outline of Chapter 12 To Clone a DNA Library? Polymerase Chain Reaction (PCR)? Making Directed Changes in the Heredity of an Organism? Plasmids Naturally occurring extrachromosomal DNA Plasmids are circular dsDNA Plasmids can be cleaved by restriction enzymes, leaving sticky ends Artificial plasmids can be constructed by linking new DNA fragments to the sticky ends of plasmid Cloning Vectors Plasmids that can be modified to carry new genes Plasmids useful as cloning vectors must have a replicator (origin of replication) a selectable marker (antibiotic resistance gene) a cloning site (site where insertion of foreign DNA will not disrupt replication or inactivate essential markers) Fig. 12-01, p.376 Fig. 12-02, p.377 Chimeric Plasmids Named for mythological beasts with body parts from several creatures After cleavage of a plasmid with a restriction enzyme, a foreign DNA fragment can be inserted Ends of the plasmid/fragment are closed to form a "recombinant plasmid" Plasmid can replicate when placed in a suitable bacterial host See Figure 12.3 12-CO, p.375 Fig. 12-03, p.378Fig....
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This note was uploaded on 12/12/2008 for the course WHO CARES 23948 taught by Professor Asshole during the Spring '08 term at University of Houston.

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nov11 - Outline of Chapter 12 To Clone a DNA Library?...

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