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nov13 - Review of Chapter 12 Cloning recombinant DNA...

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Review of Chapter 12 Cloning, recombinant DNA technology DNA Library Polymerase Chain Reaction (PCR)? Mutational analysis
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Review of Protein Synthesis A codon (triple nucleotide) Genetic code (continuous triplet code) The use of the codon table (genetic code) The genetic code is degenerate and “universal”
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Review of Protein Synthesis Initiation, elongation and termination Three stages of protein synthesis
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Review of Protein Synthesis Elongation: complex, EFs and aa-tRNAs The molecules and actions in each stage Initiation: mRNA, ribosome subunits, Ifs, and fMtRNA Termination: complex, and RFs. GTP is required in every stage
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Stages of Protein Synthesis initiation elongation termination
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Initiation of Protein Synthesis Initiation factors mRNA Ribosome (two subunits) tRNAs
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Fig. 30-18a, p. 1006
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Fig. 30-18b, p. 1006
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Fig. 30-18c, p. 1006
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Fig. 30-18, p. 1006
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Elongation cycle elongation factors mRNA-ribosome-peptidyl-tRNA complex tRNAs
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Fig. 30-19, p. 1007
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Fig. 30-21, p. 1008
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Termination release factors mRNA-ribosome-peptidyl-tRNA complex (encounter the “stop” codon on mRNA)
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