1. Cell+Biology+Techniques

1. Cell+Biology+Techniques - Modern Cell Biology Biology...

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Biology 313: Eukaryotic Cell Biology Monique Zetka (W5/28) Francois Fagotto (N3/8) April Rose - Fridays, 4:30-5:30 in room S4/106 (april.rose@mail.mcgill.ca) Christian Shaw – Tuesdays, 10:30-11:30 in room S2/2 (christianshaw@yahoo.com) Instructors Teaching Assistants Mid-term exam 30% 11:35-12:55 Thursday, February 16 Final Exam 70% Cumulative Exams are short answer and will cover only material discussed in class (may or may not be on WebCT files). Lectures will be posted the day prior to each class. Modern Cell Biology High resolution microscopy, live imaging Genome sequencing projects Functional genomics/genetic networks/proteomics Identify or isolate proteins that are a component of a particular cellular structure or mediator of a cellular process Investigate the function Localize the protein in the cell Isolate interacting proteins The yeast Saccharomyces cerevisiae can be used as a minimal eukaryotic model 16 chromosomes 13 Mb genome 2 hour doubling time Used to study the basic processes occurring in all eukaryotic cells The nematode Caenorhabditis elegans offers a compromise between tractability and complexity 6 chromosomes 97 Mb genome size 3 day generation time Entire cell lineage known Can be used to study basic developmental mechanisms
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Drosophila melanogaster offers a model system with history, and developmental and behavioural complexity 4 chromosomes 170 Mb genome 10-12 day generation time Complex body plan that has been used to study patterning Can be used to study the molecular basis of simple behaviours Arabidopsis thaliana is the leading model system for studying plant molecular genetics 5 chromosomes 125 Mb genome 6-8 week generation time culturing is nonseasonal The mouse Mus musculus serves as the mammalian model system 20 chromosomes 3000 Mb genome size 8 week generation time 23 chromosomes 3200 Mb genome size
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Approaches to Investigating Cellular Processes Isolating cells and growing them in culture Tissue samples are digested with enzymes that digest the extracellular matrix Cells are separated based on properties of the desired cell type: microdissection from tissue slices size and density adherence to plastic affinity to matrix-coupled antibodies
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1. Cell+Biology+Techniques - Modern Cell Biology Biology...

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