Homework04

Homework04 - tyr+ aro+ slo- tyr- aro- slo+ Experiment...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
BIS101-003/Engebrecht Suggested problems and homework for week #4 Problems suggested for the whole class – these are not to be turned in, but are for practice/study aid. The following problems are suggested for viral transduction: Chapter 5 in both 8 th and 9 th edition: 17, 20, 21 Chapter 7 in 7 th edition: 21, 27, 28 The following problems are suggested for DNA structure: Chapter 7 problems in both the 8 th and 9 th edition: 16, 17, 28 Chapter 8 problems in 7 th edition: 6, 7, 12 101D assignment #4 is listed on the next page. The keys to the 101D assignments will be posted on the web so that all students can do the problems.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Homework 4 1. You are trying to determine the position on the E. coli chromosome of a new mutation that you isolated, called slo- (slow growing). You do generalized P1 transduction experiments using donor and recipient strains as shown below. You observe the following cotransduction frequencies: Donor: Recipient:
Background image of page 2
Background image of page 3
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: tyr+ aro+ slo- tyr- aro- slo+ Experiment Selected marker Unselected markers 1 tyr+ 25% aro+, 30% slo- 2 aro+ 22% try+, 80% slo- Give the order of the genes. 2. You have obtained genome nucleic acid from different sources and have determined the base content. Please identify the genome structure for the following samples. State if the genome is RNA or DNA and if is likely to be single-stranded (ss) or double-stranded (ds). State if it could be from a eukaryote and/or prokaryote and/or virus. Please make sure to give a complete answer to obtain full credit. A. 27% G; 23% A; 27% C; 23% T B. 27% G; 27% A; 23% C; 23% U C. 23% G; 27% A; 27% C; 23% T D. 27% G; 23% A; 27% C; 23% U E. You look at the denaturation kinetics of samples A and C and see the following absorbance at 260nm (on next page). Please label which line corresponds to sample A and which to sample C. A 1.0 65 70 75 80 85 90 95 Temperature ( o C)...
View Full Document

This note was uploaded on 12/23/2008 for the course BIS 101 taught by Professor Simonchan during the Fall '08 term at UC Davis.

Page1 / 3

Homework04 - tyr+ aro+ slo- tyr- aro- slo+ Experiment...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online