Homework04key

Homework04key - State if the genome is RNA or DNA and if is...

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1. You are trying to determine the position on the E. coli chromosome of a new mutation that you isolated, called slo- (slow growing). You do generalized P1 transduction experiments using donor and recipient strains as shown below. You observe the following cotransduction frequencies: Donor: Recipient: tyr+ aro+ slo- tyr- aro- slo+ Experiment Selected marker Unselected markers 1 tyr+ 25% aro+, 30% slo- 2 aro+ 22% try+, 80% slo- Give the order of the genes. The order must be: tyr slo aro (or aro slo tyr) 2. You have obtained genome nucleic acid from different sources and have determined the base content. Please identify the genome structure for the following samples.
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Unformatted text preview: State if the genome is RNA or DNA and if is likely to be single-stranded (ss) or double-stranded (ds). State if it could be from a eukaryote and/or prokaryote and/or virus. Please make sure to give a complete answer to obtain full credit. A. 27% G; 23% A; 27% C; 23% T dsDNA, eukaryote/prokaryote/virus B. 27% G; 27% A; 23% C; 23% U ssRNA virus C. 23% G; 27% A; 27% C; 23% T ssDNA virus D. 27% G; 23% A; 27% C; 23% U dsRNA virus E. You look at the denaturation kinetics of samples A and C and see the following absorbance at 260nm. Please label which line corresponds to sample A and which to sample C. A 1.0 65 70 75 80 85 90 95 Temperature ( o C) C A...
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