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Unformatted text preview: also measured in the mutant strains, where + = accumulation and indicates no significant accumulation. Growth on metabolites Maximal accumulation of metabolites A B C D E F A B C D E F Mutant 1 + + - - - - - - + - - - 2 - + - - - - + - - + - - 3 + + + - - - - - - - + - 4 - + - + - - - - - - - + 5 - + - + - - - - - - - + Draw a map of the biochemical pathway, with intermediate metabolites in their proper relationship to one another, and the position of each mutation in the pathway. Mutants 4 and 5 were not distinguishable from each other in the above assays. Please indicate the three possible relationships these mutants have. How would you distinguish between these possibilities? (For each possibility indicate if complementation between mutant 4 and 5 is expected, and whether any intragenic recombination between 4 and 5 is expected)....
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- Fall '08