Homework05

Homework05 - also measured in the mutant strains, where + =...

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BIS101/Engebrecht Homework05 Recommended Chapter problems: Chapter 7: 21, 23 in both 8 th and 9 th edition (no corresponding problem in 7 th edition) Chapter 6: 50 in both 8 th and 9 th edition (Chapter 9, 14 in 7 th edition) 1. What would be the consequence at the restrictive temperature of a temperature sensitive mutant in polI that completely inactivates the protein? Please draw what a fork would look like in the mutant. Be sure to label your picture with the polarity of the strands and what everything is and indicate the defect.
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Challenge Question: 2. Mutants of Neurospora were selected on the basis of their requirement for compound B. The following data were obtained from growth tests in which the intermediate metabolites A, C, D, E, and F are supplied singly (all of the metabolites are stable and can enter the cell). + indicates growth and – indicates no growth. Accumulation of the metabolites were
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Unformatted text preview: also measured in the mutant strains, where + = accumulation and indicates no significant accumulation. Growth on metabolites Maximal accumulation of metabolites A B C D E F A B C D E F Mutant 1 + + - - - - - - + - - - 2 - + - - - - + - - + - - 3 + + + - - - - - - - + - 4 - + - + - - - - - - - + 5 - + - + - - - - - - - + Draw a map of the biochemical pathway, with intermediate metabolites in their proper relationship to one another, and the position of each mutation in the pathway. Mutants 4 and 5 were not distinguishable from each other in the above assays. Please indicate the three possible relationships these mutants have. How would you distinguish between these possibilities? (For each possibility indicate if complementation between mutant 4 and 5 is expected, and whether any intragenic recombination between 4 and 5 is expected)....
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Homework05 - also measured in the mutant strains, where + =...

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