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Homework05key - BIS101/Engebrecht Homework05 1 What would...

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BIS101/Engebrecht Homework05 1. What would be the consequence at the restrictive temperature of a temperature sensitive mutant in polI that completely inactivates the protein? Please draw what a fork would look like in the mutant. Be sure to label your picture with the polarity of the strands and what everything is and indicate the defect. Because polI provides the exonuclease to remove the RNA primers, these will remain in the mutant. Leading strand RNA primer 3’ 5’ 5’ 3’ 5’ 3’ 5’ Lagging strand Okazaki fragments 3’ =
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1. Mutants of Neurospora were selected on the basis of their requirement for compound B. The following data were obtained from growth tests in which the intermediate metabolites A, C, D, E, and F are supplied singly (all of the metabolites are stable and can enter the cell). + indicates growth and – indicates no growth. Accumulation of the metabolites were also measured in the mutant strains, where + = accumulation and – indicates no significant accumulation. Growth on metabolites Maximal accumulation of metabolites
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