ELN MCB3020L.docx - Life in a Drop of Water Phylogenic and...

Info icon This preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
Life in a Drop of Water, Phylogenic and Microscopic Examination of Wet Mount (Module 1) Purpose: Purpose of this experiment was to develop skills in identifying microorganism phylogenies in wet mount from pond water, and to further develop skills in microscope usage, for future experiments. Materials : 1 Microscope Microscope Slides Microscope Slide covers Small jar containing murky, colored water Transfer Pipette Computer with Virtual Ponddip Application Procedures : 1. Turn on the microscope, and set to the lowest magnification 4x and 10x objective 2. Get one microscope slide, and with the transfer pipette, obtain water from the small jar 3. Add one drop of water from the pipette to the microscope slide, and then cover the slide with a slide cover 4. Place the slide over the light source on the microscope, and begin observing and specimens present. 5. Increase magnification, to better see any organisms. 6. Identify 4 different specimens using the Virtual Ponddip application on the computer Results : Name: Genus: Volvox Volvox is classified as green algae, but others would classify it as a protozoan. Volvox can consist of hundreds of other volvox microorganisms, and within the Volvox itself, there is can be small colonies. Volvox can be found on the surface of flowing water. They also have two flagella to help with motility.
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Name (Genus): Amoeba Amoebas are unicellular protists, which use their protrusions, for movement. Amoebas flow over a surface, turning from a liquid to a solid state. They surround and engulf their food during feeding Name (genus): Actinospaehrium They have protrusions of cytoplasm that they use to hunt and capture prey. They are considered protists. They are generally found with plants. Name (genus): Cypris Their bodies is enclosed by a shell.Although not shown by the picture, they have antennas and limbs. They are generally found with aquatic vegetation.
Image of page 2
Interpretation : This experiment revealed that even within a small drop of water, there are plenty of microorganisms living within it. This experiment also revealed that these microorganisms each have differing shapes and characteristics. My results say about my samples, is that they are standard to be seen within a murky water sample. This exercise helped, to get familiarized with the microscope, and identifying organisms. Glossary 1. Amoeba: Cell with ability to change its shape, and how protruding limbs 2. Algae: Eukaryotic, have chlorophyll and does photosynthesis. 3. Phylogeny: The evolutionary history of an organism 4. Paramedium:genus of ciliated protozoa, live in freshwater 5. Taxonomy: The classification, and ordering of organisms 6. Extremophile: Organism that can live in extreme conditions 7. Diatom: Major group of algae, shaped like zigzags, or fans 8. NCBI:National Center of Biotechnology Information 9. Rotifer: microscopic animals, usually inhabit freshwater 10. Protozoa: unicellular eukaryotic, animal like behavior Lab 2: Collecting Microbes samples for unknown and gram staining, pipetting (module 2) Purpose:
Image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern