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Lab report.docx - Isolating Chlorophyll and Carotenoid...

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Isolating Chlorophyll and Carotenoid Pigments from Spinach The purpose of this experiment is to separate the chlorophyll and carotenoid pigments from fresh spinach leaves. The spinach is separated through the process of column chromatography and is then tested with thin-layer chromatography. All the pigments that are extracted are nonpolar, however, the chlorophyll are relatively more polar than the carotenes. Beta carotene are hydrocarbons, which makes them nonpolar. Chlorophyll has polar bonds to magnesium and more bonds to polar functional groups, this makes it significantly more polar than the carotenoids. This allows for accurate separation with solvents increasing in polarity to extract out each band successfully. The carotenoids are yellow pigments that contain beta- carotene, alpha-carotene, and xanthophylls. The carotenes move faster and easier down the column. The chlorophyll in the spinach comes in two forms, chlorophyll A and chlorophyll B.
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