Isolating Caffeine from Tea Leaves.docx

Isolating Caffeine from Tea Leaves.docx - Isolating...

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Isolating Caffeine from Tea Leaves The purpose of this experiment is to isolate caffeine from tea leaves using extraction and sublimation. The structure of caffeine is similar to that of purines in DNA, which is what causes the caffeine to affect biological pathways in the body. The process of the extraction is summed up below: Tea (basic extraction with sodium carbonate) Organic Layer (CH 2 Cl2) Basic Aqueous Layer Soluble Salts of Hydrolyzed Tannins When transferring the tea to the centrifuge tubes, it was observed that the tea was too dilute and had too much water. This meant that the tea would not fit equally into two centrifuge tubes; instead, four centrifuge tubes were used. Although four centrifuge tubes were used, the same amount of sodium carbonate and methylene Ionic and soluble in water
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chloride were used. The percent recovery of the caffeine prior to sublimation was 97.39%. Some yield could have been lost when evaporating the methylene chloride
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