098469.pdf - ear = 469 fli£ifik$98¥$fizfi flan...

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Unformatted text preview: ear = 469 fli£ifik$98¥$fizfi flan ia‘fisfifl Ha : aaaaaw) aa=469 - EEWEWEP ’ attenuation figfiflfilflfififig—‘Efiib fighflfifififlflifia‘z attenuation Eglfiifill ' fifi'flifigififiifilfififfigfifififiiwtp ° (10 fl) . ngmtt—EDEJI‘Z'EWEP ’ small RNA maraaamaaz gene silencing ’ Eifidiifififilfiififl *1 8 a . JEEEWEEEEXE} sigma factor Efigfi ’EIEEEEZWE sigma factors ° Efi‘é‘fiEfi sigma faCtOI‘ WfijfifififiifilfirfigfiT‘lfifig sigma faCtOIS? _( 5 fi) . fimgtpaufipa DNA replication E’s Fess : (1) DNA polymerases WWEEIEEEB’J nucleotides? ( 4 6}) (2) DNA replication fillfifig primers? Eiifijiggiifiilé‘iimtlj ’ filmgfid RNA “5% primer ! fi’fifiqfl DNA? (5 53‘) (3) EWEW?HW%$UE*7X €611 CYCIB Killifii—‘W DNA replication? (6 fl) . fig doubleastrand break-repair model fig homologous recombination Hfiififi ’Ifl'igifiwg homologous recombination Efifiiwtllfififlfifigbyjfifi o ( 10 fl) . fiflfi‘l‘flfififi DNA repair E55233 1 (filbflg 3 5}) (1) photoreactivation (2) translesion DNA synthesis (3) glycosylase (4) mismatch repair 7. Please explain the similarities and differences of DNA replication and transcription in detail. (10 ii) 8. There are three classes of RNA splicing, nuclear pre—mRNA, Group II introns and Group I introns. Please explain their mechanism and catalytic machinery in detail. (10 53‘) 9. Please explain the principles of the following experimental methods schematically (draw pictures with detail explanation). And what are these methods used for? (10 fi) (1) EMSA (electrophoretic mobility—shift assay) (2) nuclease protection footprinting (3) SELEX (systematic evolution of ligands by exponential enrichment) (4) ChIP (chromatin immunoprecipitation) 10. Arabidopsis and Caenorhabdiris elegans are often viewed as model organisms. Please explain why? Use the following features or terms appropriately. T-DNA, reverse genetics, tilling, small genome, simple body plan, cell death pathway, rapid life cycle, dauer. (10 ’53) aaaaaa ...
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