Lecture 05 2B03 2017.pptx - Lecture 5 DNA replication...

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Based on Chapter 28: DNA Metabolism: Replication, Recombination, and Repair Lecture 5 DNA replication, recombination and repair Part I 1
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Additional learning video Copy and paste this link to see a video on DNA replication for further learning https :// www.youtube.com/watch?v=27TxKoFU2Nw
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Key Features of DNA Replication in Prokaryotes Key Features of DNA Replication in Prokaryotes Semiconservative one of the 2 strands is conserved in each progeny molecule Bidirectional it proceeds in both directions from the same starting point (origin of replication) oriC in E. coli Semi-discontinuous - the lagging strand is made via Okazaki fragments, then joined 3
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Bidirectional Replication Bidirectional Replication It involves two replication forks , which move in opposite directions 4 Replication fork 1 Replication fork 2 oriC oriC
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The replication fork 5’ 3’ 5’ 3’ 5’ 3’ DNAP DNAP DNAP (DNA Polymerase) 2 questions: 1. How can 2 antiparallel DNA strands extend in the same replication fork if DNAP can only extend 5’ 3’? 2. How can DNAP begin replication if it needs a preexisting 3’ end?
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DNA replication is: 3. Semi discontinuous - the lagging strand is made via Okazaki fragments, then joined 5’ 3’ 5’ 3’ 5’ 3’ DNAP DNAP DNAP (DNA Polymerase) Leading strand = parental strain in the 5’ 3’ direction Leading strand replication – continuous extension in the direction of fork movement Lagging strand = parental strain in the 3’ 5’ direction Lagging strand replication – discontinuous extension in the direction opposite to the direction of fork movement Okazaki fragments 2 questions: 1. How can 2 antiparallel DNA strands extend in the same replication fork
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