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Unformatted text preview: width 3 472 Eiifik$98$$§lfiffifliagtfifififl fie =fiiith$(E) steam ' at 3 Exit! / ‘a’ a—se 1- Egg??? 3 (24%) l. Quorum sensing . Stationary phase . Sigma factor Conjugation Auxotroph Lactic acid fermentation . lcosahedral capsids . F plasmid II. figfi: (10%) 1. Please give an example to describe the measurement of bacterial growth numbers. 2. Please describe two chemical agents in terms of mechanism of action that can be used to control microorganisms. HI- E—iEvEE: (16%) X unease ré‘Fififiafi’i’FgEJ fiflflfifitififii’fig ° 1. 0.2 mls of a 10'4 dilution of a virus preparation yields 90 plaques. What is the number of PFU per ml in the undiluted virus preparation? A. 9.0 x105 B. 4.5 ><106 C. 4.5 x107 D. 9.0x 108 2. Bacteriophage that only exhibit lytic replication cycles are called phages. A. temperate B. virulent C. intemperate D. lytigate 3. Which of the following has been associated with a form of liver cancer? A. human papillcma virus B. hepatitis B virus C. human T-cell lymphotropic virus . D. hepatitis A virus 4. Which of the following does not use RNA as the genome? A. adenovirus B. poliovirus C. influenza virus D. rabies virus 5. T ransposons that transpose by inserting a copy at a new location while a copy remains at the original location are said to transpose by transposition. A. simple B. composite C. incomplete D. replicative 6. The Ames test A. can be used to measure the mutagenicity of chemicals. B. is used to measure the repair of thymine dimers. C. is used to measure levels of oxygen free radicals. D. none of the choices 7. During sporulation in Bacillus subtilis, inactive precursor fonns of new sigma factors are activated at the appropriate time by A. proteolysis B. RNA binding C. phosphorylatiou D. chaperone re-folding 8. If Escherichia coli is cultured in broth containing both glucose and lactose, it A. uses glucose preferentially until the supply is exhausted, then uses lactose. B. uses lactose preferentially until the supply is exhausted, then uses glucose. C. uses glucose and lactose simultaneously. D. uses only the glucose (it cannot use lactose as a source of carbon). .Bfifi 235% 3 472 BiiflkfiQB'fifi-fiififflfiii‘flfififi it‘tfi ”taillafiCE) steam at 3 its 2 a _ ‘ seas (slate ’ a-zoa) >:< snares: Pineal/rate. sang .. l. Archaeal membranes contain which of the following polar lipids? A. phospholipids B. sulfolipids C. glycolipids D. all of these 2. Methanogens can live only in environments. A. aerobic B. anaerobic C. microaerophilic D. all of these 3. Cyanobacteria are best described as A. all being obligate photolithoautotrophs. B. all being obligate chemoheterotrophs. C. some being photolithoautotrophs that can fimction as chemoheterotrophs in the dark. D. none of the choices 4. Helicobacter pylori is responsible for A. pneumonia. B. cholera. C. dysentery. D. peptic ulcer disease. 5. The process of conversion of ammonia to nitrate is referred to as A. nitrogen fixation. B. nitrification. C. ammonification. D. denitrification. 6. Which of the following is associated with dental caries? A. Streptococcus pneumoniae B. S. mutans C. E. fecaelis D. Lactococcus lactis 7. Which of the following members of the genus Bacillus is currently used as a biological insecticide? A. B. cereus B. B. subtilr's C. B. anthracis D. B. fhuringiensis 8. Actinomycetes are usually found in habitats. A. soil B. freshwater C. marine . D. all of the choices 9. The body or vegetative structure of a fiingus is called a A. mold. B. thallus. C. hypha. D. yeast. 10, In fungi, spores produced by budding are called A. blastospores. B. conidiospores. C. arthrospores. D. sporangiospores. 11. The primary storage polysaccharide in fiingi is A. glycogen. B. cellulose. C. starch. D. none of the choices 12. A major fully reduced form of carbon, important in biogeochemical cycling is A. carbon dioxide. B. carbohydrate. C. methane. D. hydrogen sulfide. 13. When microorganisms such as algae use dissolved carbon dioxide present in freshwater ecosystems, the water pH will A. increase. B. decrease. C. not be changed. D. first increase then decrease. . A mature activated B cell is called a(n) A. plasma cell. B. dendritic cell. C. natural killer cell. D. spleen cell. . Which of the following is a physical barrier in the nonspecific defense of a mammalian host? A. inflammation B. phagocytosis C. fever D. mucous membranes . The alternate complement pathway plays an important role in A. innate immunity. B. aquired immunity. C. specific immunity. D. both innate immunity and specific immunity. 17. The characteristics of a pathogen that determine its virulence include which of the following? A. patho genicity B. invasiveness C. infectivity D. all of the choices ear: 333% 1 472 @iifik$98$$fififi :lrliiia'i#éififli are iiiiiilhfiw) stem at 3 Ea? 3 E 18. Ti DNA of the genus Agrobacrerium A. is required for nitrogen fixation. B. has been used to construct genetically engineered plants. C. carries the genes needed for root nodule development. D. carries the genes needed for formation of bacteroids. 19. T cells produce and secrete factors which do not directly interact with invading microorganisms but which augment the body's defense mechanisms. These molecules are called A. antibodies. B. cytokines. C. immunogens. D. augmetins. 20. A plasmid bearing one or more antibiotic resistance genes is a(n) A. F plasmid. B. A plasmid. C. R plasmid, D. D factor. :_ ~ nemeeaaeseee ’ EriES’J} ’ ) i. Exotoxin A. Water soluble I. Direct contact éHOéa‘) ) 2. Endotoxin B. Water insoluble J. Indirect contact C. Glycoprotein K. Weakly immunogenic D. Lipoprotein L. Highly immunogenic E. Heat-stable M. Glycopolysaccharide F. Heat-labile N. Lipopolysaccharide G. Species specific 0. Xenobiotic H. Non—specific P. Gnotobiotic 5- ‘ éfilififi = TE]Eifiiiafififlfiefifiéfifikfié‘mfléiifiifiifii’efi‘ifciifiifi ’ %% EfifiJfiEififiéfii’Ffli/fiifi ° (fiie-Zé} ’ vii-10%) Anaerobic Aerobic Carbon fixation Organic matter (CH9) co 3. Anaerobic resPiration and ( Carbon monoxide oxidation 00 [,3 \ gg—fiflgfiafijfiifibfifi: @7353] fléflgreen gene technologyfltfii Eii'éifi ‘ fia\%g%§ci #éJ’Eii'fii—Pfiiéiifiéfi % é ’ fitiiffifi Efififififiiflfléfifilifi ° (10%) aeseae ...
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