Homework Resource Week 9.docx - Brigham Young University...

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Brigham Young University — Idaho Bio 180 Lab Lab 9 Homework: Enzyme Kinetics Lab Packet 1. Use the data from your lab to create a Bar Graph. There will be 6 bars on the graph, the first 2 will show the cold temperature and the cold temperature moved to 32, the next 2 bars will show 32 and then moved to 32, and the final 2 bars will show hot temperature and hot moved to 32 degrees (only use the average enzyme activity for your bars). Please insert Bar Graph below: 1.5 32 60 1.5-32 32-32 60-32 0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45 0.5 Temperature Absorption/minute Average 2. Which temperature irreversibly denatures peroxidase? How does your data indicate that the enzyme is irreversibly denatured? The temperature that irreversible denatures peroxidase is the 60 degrees one. Because my data indicates that at 60 degrees, there is no reaction and even if we put it back to normal (60-32 degrees), it still doesn’t seem to react, it was below 0.02 degree, which means it don’t even want to react, or it react really slowly, doesn’t seem to have much reaction, this means some of the bonds was being broke and irrevered.
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  • Spring '16
  • Holyoch

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