Lab 08 HW.docx - Methods To determine the solution of the...

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Methods To determine the % solution of the unknown, use multiple % solutions of glucose, a spectrometer, and the benedict’s test to create a linear fit line. First, create 5 different % solutions of glucose using the 2% solution and diluting it into a 0, 0.5, 1, 1.5, and 2% by adding the appropriate amount of distilled water to each solution as shown below to create 4ml of solution. Then take 2ml of each solution and put it into its own labeled beaker. Add 1 ml of Benedict’s solution to each one, stir it, and put it in boiling water for 3 mins. After 5 minutes of cooling, put each solution through the spectrometer and record the data and create a linear fit line using Logger Pro. In Logger Pro, the axis will be labeled ‘Percent Solution’ for the x and ‘absorption’ for y. The spectrometer will be calibrated to distilled water. Do the Benedict’s test again for the unknown solution and put it through the spectrometer. Using the collected data and the linear fit line previously determined I will calculate the exact % solution of glucose in the unknown solution.
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