Outline 3.doc - Microscopy ,i.e.magnification o...

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Microscopy Function of Microscopes Microscopes produce an enlarges image of object, i.e. magnification o Length of smallest object seen by human eye ~ 0.2 mm ( Fig 2.7 ) Two broad classes of microscopes o Simple: single lens o Compound: more than one lens  o Mag. Power is related to the FOCAL LENGTH (f) of a lens: Small f (fat lens) = high mag. Long f (thin lens) = low mag. “Strength” of a microscope is known as its MAGNIFYING POWER Common types of microscopes o Light microscopes: uses light to produce image o Electron microscopes: uses electrons to produce image Quantitative Indices of Microscope Function Indices of Microscope Function 1. Total Magnification: Objective mag. (4X, 10X, 40X) x Eyepiece mag. (10X) 2. Resolution  a. Ability to distinguish two very close objects as distinct entities i. Objective lens very important for good resolution since objective  must produce a clear and magnified image ii. Measured as the minimum distance (d) between two objects that  reveal them as separate entities  d = 0.5(lambda)/ NA (slide 3)   - memorize formula -
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