Outline 3.doc - Microscopy ,i.e.magnification o...

Info icon This preview shows pages 1–2. Sign up to view the full content.

Microscopy Function of Microscopes Microscopes produce an enlarges image of object, i.e. magnification o Length of smallest object seen by human eye ~ 0.2 mm ( Fig 2.7 ) Two broad classes of microscopes o Simple: single lens o Compound: more than one lens  o Mag. Power is related to the FOCAL LENGTH (f) of a lens: Small f (fat lens) = high mag. Long f (thin lens) = low mag. “Strength” of a microscope is known as its MAGNIFYING POWER Common types of microscopes o Light microscopes: uses light to produce image o Electron microscopes: uses electrons to produce image Quantitative Indices of Microscope Function Indices of Microscope Function 1. Total Magnification: Objective mag. (4X, 10X, 40X) x Eyepiece mag. (10X) 2. Resolution  a. Ability to distinguish two very close objects as distinct entities i. Objective lens very important for good resolution since objective  must produce a clear and magnified image ii. Measured as the minimum distance (d) between two objects that  reveal them as separate entities  d = 0.5(lambda)/ NA (slide 3)   - memorize formula -
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

Image of page 2
This is the end of the preview. Sign up to access the rest of the document.
  • Fall '08

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern