LAB2.docx - NAME Varsha Sooklal ID 66280 LAB 2 TITLE Red Blood Cell Count Using a Hemacytometer INTRODUCTION Sufficient numbers of red blood cells(RBC

LAB2.docx - NAME Varsha Sooklal ID 66280 LAB 2 TITLE Red...

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NAME: Varsha Sooklal ID: 66280 LAB# 2 TITLE: Red Blood Cell Count Using a Hemacytometer INTRODUCTION: Sufficient numbers of red blood cells (RBC, or erythrocytes) are necessary for adequate transport of oxygen from the lungs to the peripheral tissues. Too few RBCs constitutes a pathological condition known as anemia (lit., without blood). According to the Merck Manual, normal values of RBC/cmm for males is 5.4 + 0.8 million, and 4.8 + 0.6 for females. Anemic levels for adult males are below 4.5 million, for females below 4.0 million. We will perform red and white blood cell counts on your blood in the lab using a hemocytometer and appropriately diluted blood. Blood cell counts can be performed using the hemacytometer. This precision instrument possesses a platform with microscopic grid scoring. Rails on either side hold up a cover slip so that a specified quantity of fluid is held. By properly diluting blood, counting all cells in specified squares, and multiplying by the proper conversion factor, the number of cells per cubic millimeter can be determined. AIM: To determine the red and white blood cells count in four individuals using a hemacytometer
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METHODOLOGY: Materials/Equipment: 1 hemacytometer 2 coverslips 2 lancets needless (for drawing blood) 2 glass tubes 1 250 ml beaker for waste fluid 1 250 ml beaker for waste tips 1 bottle WBC diluents (purple) 1 bottle RBC diluents (clear) 6 alcohol swabs 2 paper towels 1 20-200µl pipette 1 1 ml (1000µl) pipette 1 set of 20µl pipette tips 1 set of 1 ml pipette tips
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PROCEDURE: Red and White Blood Cell Count 1. All equipment was set up on the desk so that we were sure to have had everything ready for the procedure. 2. 1980µl of the diluent solution (ringer’s solution for RBC and purple solution for WBC) was pipette into a small glass tube. (dilution of 1 to 200) 3. Alcohol was then used to swab towards the side of the tip of a little-used finger.(Not close to the cuticle) 4. The side of the finger was then lanced with a quick and firm jab, wiping away the first blood using alcohol swabs. 5. 20 µl of blood was drawn up. This was done by slightly slanting the pipette down to allow the blood to flow in. 6. 20 µl of blood was emptied into the premeasured diluents solution tube.
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