NAME: Varsha Sooklal ID: 66280 TITLE: Orientation Lab INTRODUCTION: One was required to visit a number of stations of which knowledge would be gained on using micropipettes, spectrophotometers, microscopes, measuring pH, the BME (Biomedical Engineering) Lab facilities as well as laboratory safety equipment. AIM: To gain knowledge on the use of micropipettes, spectrophotometers, measuring pH, the BME lab facilities and laboratory safety equipment. METHODOLOGY: Materials/Equipment: Station 1 0.01% Xylene Cyanol Micropipettes – P20, P200, P1000 Distilled water Eppendorf tubes Station 2 Specord 210 Spectrophotometer Cuvettes Station 3 Digital microscope Station 4 0.01M HCl, 0.1M NaOH, 0.0001M HCl, 1M NaOH Kimwipes Distilled Water
Calibration buffer pH meter – electrode Beaker Station 5 Laminar flow (biosafety cabinet), gel electrophoresis tank, micro plate washer CO 2 tank, micro plate reader, gel documentation station Incubator, centrifuge, PCR Station 6 Safety shower, fire blanket, Spill kits, Eyewash stations, chemical disposal PROCEDURE: Station 1: Introduction to pipetting Selecting the Appropriate Tip and using it to transfer liquid 1. A tip was placed on the micropipette using the appropriate tip size. 2. When transferring the liquid, the plunger was pressed down to the first stop and then the tip was inserted into the liquid. 3. The thumb was carefully and slowly lifted to release the plunger thereby drawing the liquid up into the tip. 4. The tip was removed from the liquid and moved to the receiving tube or parafilm where the plunger was pressed to expel the liquid. The plunger was depressed to the second stop position to remove all the liquid from the tip. 5. The tip was ejected into the waste container. Using 0.01% Xylene Cyanol 1. 800 l, 80 l and 8 l of the 0.01% XC stock were pipetted three times into eppendorf tubes as a practice. 2. Using the P20, 10, 15 and 20 l of the 0.01% XC stock solution was measured into the bottom of the three eppendorf tubes. Using the P1000, water was added to bring the final volume to 1ml. 3. Using the P200, 20, 50 and 100 l of the 0.01% XC stock solution was measured into the bottom of the three eppendorf tubes. Using the P1000, water was added to bring the final volume to 1ml.
- Summer '15