Potato Osmosis Potential Lab Write Up.pdf - I Potato Osmosis Potential Lab II McNeil Beasley Julia Johnson Caroline Mangum Ashley Vincent III IV Mrs

Potato Osmosis Potential Lab Write Up.pdf - I Potato...

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Unformatted text preview: I. Potato​ ​Osmosis​ ​Potential​ ​Lab II. McNeil​ ​Beasley*,​ ​Julia​ ​Johnson,​ ​Caroline​ ​Mangum,​ ​Ashley​ ​Vincent III. September​ ​22,​ ​2017 IV. Mrs.​ ​Hodge:​ ​2nd​ ​Period V. Materials​ ​and​ ​Quantities: ● 6​ ​Beakers ● 5​ ​Colors​ ​of​ ​Dye ● 6​ ​Unknown​ ​Measurements​ ​of​ ​Sucrose​ ​(measured​ ​by​ ​teacher) ● 600mLs​ ​of​ ​Water ● 24​ ​Red​ ​Potato​ ​Bores ● Potato​ ​Borer ● Paper​ ​Towels ● Scale VI.​ ​Experimental​ ​Procedure: 1. Fill​ ​six​ ​beakers​ ​with​ ​100​ ​ml​ ​of​ ​water​ ​with​ ​food​ ​coloring​ ​to​ ​distinguish​ ​the​ ​groups (containing​ ​an​ ​unknown​ ​amount​ ​of​ ​sucrose​ ​measured​ ​by​ ​teacher)​ ​each. 2. One​ ​of​ ​the​ ​beakers​ ​will​ ​contain​ ​plain​ ​distilled​ ​water 3. Use​ ​Borer​ ​to​ ​create​ ​24​ ​potato​ ​bores 4. Cut​ ​the​ ​bores​ ​so​ ​each​ ​is​ ​the​ ​same​ ​length 5. Separate​ ​the​ ​potato​ ​bores​ ​into​ ​six​ ​groups​ ​of​ ​four 6. Use​ ​a​ ​scale​ ​to​ ​find​ ​the​ ​initial​ ​mass​ ​of​ ​ ​each​ ​group​ ​of​ ​(4)​ ​bores,​ ​and​ ​document​ ​the data 7. Place​ ​the​ ​four​ ​bores​ ​into​ ​one​ ​of​ ​the​ ​beakers​ ​and​ ​repeat​ ​for​ ​each​ ​group​ ​with​ ​a new​ ​beaker 8. Based​ ​on​ ​the​ ​amount​ ​of​ ​bores​ ​that​ ​float​ ​in​ ​the​ ​solution​ ​calculate​ ​which​ ​beaker has​ ​each​ ​amount​ ​of​ ​solute.​ ​If​ ​the​ ​bores​ ​float​ ​there​ ​is​ ​more​ ​solute​ ​and​ ​vise​ ​versa (based​ ​on​ ​our​ ​observations​ ​distilled:​ ​0m,​ ​purple:​ ​0.2​ ​M,​ ​blue:​ ​0.4​ ​M,​ ​green:​ ​0.6 M,​ ​red:​ ​0.8​ ​M,​ ​and​ ​yellow​ ​1.0​ ​M) 9. Leave​ ​the​ ​bores​ ​in​ ​the​ ​solution​ ​for​ ​48​ ​hours​ ​undisturbed 10. Drain​ ​each​ ​beaker​ ​and​ ​use​ ​a​ ​paper​ ​towel​ ​to​ ​dry​ ​the​ ​bores​ ​and​ ​remove​ ​any excess​ ​water 11. Use​ ​the​ ​scale​ ​to​ ​find​ ​the​ ​final​ ​mass​ ​of​ ​each​ ​group​ ​of​ ​bores​ ​(4)​ ​and​ ​document​ ​the data 12. Finally,​ ​calculate​ ​the​ ​difference​ ​between​ ​the​ ​initial​ ​and​ ​the​ ​final​ ​mass​ ​of​ ​each group,​ ​the​ ​percent​ ​of​ ​change,​ ​and​ ​whether​ ​the​ ​cell​ ​to​ ​the​ ​solution​ ​is​ ​hypertonic​ ​or hypotonic VII.​ ​Purpose The​ ​purpose​ ​of​ ​the​ ​experiment​ ​is​ ​to​ ​study​ ​osmosis​ ​and​ ​test​ ​the​ ​facilitated​ ​transport​ ​of water​ ​through​ ​a​ ​cell​ ​membrane. VIII.​ ​Hypothesis If​ ​the​ ​molarity​ ​of​ ​sucrose​ ​increases​ ​in​ ​a​ ​solution​ ​then​ ​the​ ​potato​ ​bores​ ​will​ ​go​ ​from hypertonic​ ​to​ ​hypotonic. Pre-Experiment​ ​Molarity​ ​Predictions: Distilled:​ ​0.0​ ​M Purple:​ ​0.2​ ​M Blue:​ ​0.4​ ​M Green:​ ​0.6​ ​M Red:​ ​0.8​ ​M Purple:​ ​1.0​ ​M IX. Solution Picture Observations Distilled​ ​Water​ ​ ​Solution Before​:​ ​Most​ ​Potatoes sank​ ​in​ ​the​ ​solution. After​:​ ​Potatoes sponge-like​ ​and​ ​potato bores​ ​expanded​ ​and became​ ​turgid. Purple​ ​Solution Before​:​ ​Most​ ​Potatoes sank​ ​in​ ​the​ ​solution. After​:​ ​Potatoes sponge-like,​ ​potato​ ​bores expanded​ ​and​ ​became turgid. Blue​ ​Solution Before​:​ ​Most​ ​Potatoes float​ ​in​ ​the​ ​solution. After​:​ ​Potatoes sponge-like,​ ​change​ ​in​ ​the volume​ ​and​ ​mass​ ​of​ ​potato was​ ​minimal. Green​ ​Solution Before​:​ ​Most​ ​Potatoes float​ ​in​ ​the​ ​solution. After​:​ ​Potatoes sponge-like,​ ​Potato​ ​bores lost​ ​volume​ ​and​ ​mass​ ​and became​ ​flaccid. Red​ ​Solution Before​:​ ​Most​ ​Potatoes float​ ​in​ ​the​ ​solution. After​:​ ​Potatoes sponge-like,​ ​Potato​ ​bores lost​ ​volume​ ​and​ ​mass​ ​and became​ ​flaccid. Yellow​ ​Solution Before​:​ ​Most​ ​Potatoes float​ ​in​ ​the​ ​solution. After​:​ ​Potatoes sponge-like,​ ​ ​Potato​ ​bores lost​ ​volume​ ​and​ ​mass​ ​and became​ ​flaccid. X. Solution Initial​ ​Mass Mass​ ​After Difference​ ​in Mass Distilled Water 2.3729​ ​g 3.115​ ​g (+)​ ​0.7421g 31.274% Increase Hypertonic Purple Solution 1.8432​ ​g 2.040​ ​g (+)​ ​0.1968 10.677% Increase Hypertonic Blue Solution 2.0262​ ​g 1.921​ ​g (-)​ ​0.1052 5.192% Decrease Hypotonic Red Solution 2.2610​ ​g 1.897​ ​g (-)​ ​0.3640 16.099% Decrease Hypotonic Green Solution 2.3587​ ​g 1.717​ ​g (-)​ ​0.6417 27.206% Decrease Hypotonic Yellow Solution 2.6250​ ​g 1.670​ ​g (-)​ ​0.9550 36.681% Decrease Hypotonic %​ ​ ​ ​in mass Cell​ ​to​ ​the Solution XI.​ ​ ​ ​ ​Part​ ​One:​ ​Our​ ​hypothesis​ ​was​ ​correct​ ​because​ ​the​ ​potatoes​ ​that​ ​were​ ​in​ ​the higher ​ ​ ​ ​ ​ ​ ​ ​ ​concentration​ ​of​ ​sucrose​ ​were​ ​hypotonic​ ​while​ ​the​ ​potatoes​ ​in​ ​the​ ​lower ​ ​ ​ ​ ​ ​ ​ ​ ​concentrations​ ​were​ ​hypertonic.​ ​Our​ ​predictions​ ​of​ ​the​ ​molarity​ ​of​ ​the​ ​sucrose ​ ​ ​ ​ ​ ​ ​ ​ ​solutions​ ​were​ ​incorrect​ ​ ​because​ ​we​ ​initially​ ​believed​ ​the​ ​red​ ​solution​ ​to​ ​have​ ​a ​ ​ ​ ​ ​ ​ ​ ​ ​higher​ ​concentration​ ​than​ ​that​ ​of​ ​green,​ ​however,​ ​based​ ​off​ ​the​ ​results​ ​of​ ​the ​ ​ ​ ​ ​ ​ ​ ​ ​experiment,​ ​green​ ​appears​ ​to​ ​have​ ​a​ ​higher​ ​sucrose​ ​concentration​ ​than​ ​that​ ​of​ ​red. ​ ​ ​ ​ ​ ​ ​ ​ ​ ​Part​ ​Two:​ ​The​ ​distilled​ ​water​ ​(0.0​ ​M​ ​sucrose)​ ​was​ ​hypotonic​ ​to​ ​the​ ​potatoes ​ ​ ​ ​ ​ ​ ​ ​ ​ ​because​ ​the​ ​potatoes​ ​had​ ​an​ ​increase​ ​in​ ​mass​ ​by​ ​31.3%.​ ​The​ ​purple​ ​solution ​ ​ ​ ​ ​ ​ ​ ​ ​ ​(found​ ​to​ ​be​ ​0.2​ ​M​ ​Sucrose)​ ​was​ ​hypotonic​ ​to​ ​the​ ​Potatoes​ ​since​ ​the​ ​potato​ ​mass ​ ​ ​ ​ ​ ​ ​ ​ ​ ​increased​ ​10.7%​ ​ ​potatoes,​ ​but​ ​less​ ​hypotonic​ ​than​ ​distilled​ ​water​ ​and​ ​slight ​ ​ ​ ​ ​ ​ ​ ​ ​ ​increase​ ​in​ ​mass​ ​was​ ​observed.​ ​The​ ​blue​ ​solution​ ​(found​ ​to​ ​be​ ​0.4​ ​M​ ​sucrose) was ​ ​ ​ ​ ​ ​ ​ ​ ​ ​observed​ ​to​ ​have​ ​a​ ​decrease​ ​in​ ​mass,​ ​therefore​ ​the​ ​solution​ ​was​ ​hypertonic​ ​to​ ​the ​ ​ ​ ​ ​ ​ ​ ​ ​ ​potatoes.​ ​The​ ​red​ ​solution​ ​(found​ ​to​ ​be​ ​.6​ ​M​ ​sucrose)​ ​had​ ​a​ ​decrease​ ​in​ ​mass​ ​of ​ ​ ​ ​ ​ ​ ​ ​ ​ ​about​ ​16.1%,​ ​therefore​ ​the​ ​solution​ ​was​ ​hypertonic​ ​to​ ​the​ ​potatoes.​ ​The​ ​green ​ ​ ​ ​ ​ ​ ​ ​ ​ ​solution​ ​(found​ ​to​ ​be​ ​0.8​ ​M)​ ​had​ ​a​ ​decrease​ ​of​ ​27.2%​ ​in​ ​mass,​ ​therefore​ ​the solution ​ ​ ​ ​ ​ ​ ​ ​ ​ ​was​ ​hypertonic​ ​to​ ​the​ ​potatoes.​ ​The​ ​yellow​ ​solution​ ​(found​ ​to​ ​be​ ​1.0​ ​M)​ ​had​ ​a ​ ​ ​ ​ ​ ​ ​ ​ ​ ​36.7%​ ​decrease​ ​in​ ​mass,​ ​therefore​ ​the​ ​solution​ ​was​ ​hypertonic​ ​to​ ​the​ ​potatoes. The ​ ​ ​ ​ ​ ​ ​ ​ ​ ​results​ ​support​ ​the​ ​hypothesis​ ​because​ ​as​ ​the​ ​sucrose​ ​molarity​ ​increased​ ​the bores ​ ​ ​ ​ ​ ​ ​ ​ ​went​ ​from​ ​hypertonic​ ​to​ ​hypotonic​ ​in​ ​respect​ ​to​ ​the​ ​solution. ​ ​ ​ ​ ​ ​ ​ ​ ​ ​Part​ ​Three:​ ​No​ ​Sources ​ ​ ​ ​ ​ ​ ​ ​ ​ ​Part​ ​Four:​ ​Error​ ​may​ ​have​ ​occurred​ ​because​ ​we​ ​dropped​ ​the​ ​potato​ ​bores,​ ​that had ​ ​ ​ ​ ​ ​ ​ ​ ​ ​been​ ​placed​ ​in​ ​the​ ​blue​ ​solution,​ ​on​ ​the​ ​ground.​ ​This​ ​could​ ​have​ ​caused​ ​an ​ ​ ​ ​ ​ ​ ​ ​ ​ ​inaccurate​ ​final​ ​mass​ ​of​ ​the​ ​potato​ ​bores ​ ​ ​ ​ ​ ​ ​ ​ ​ ​Part​ ​Five:​ ​To​ ​eliminate​ ​any​ ​error,​ ​we​ ​could​ ​be​ ​more​ ​precise​ ​and​ ​careful​ ​in handling ​ ​ ​ ​ ​ ​ ​ ​ ​ ​the​ ​materials​ ​and​ ​variables​ ​of​ ​the​ ​experiment. ​ ​ ​ ​ ​ ​ ​ ​ ​Part​ ​Six:​ ​To​ ​further​ ​our​ ​experiment,​ ​we​ ​could​ ​use​ ​different​ ​types​ ​of​ ​potatoes because ​ ​ ​ ​ ​ ​ ​ ​ ​we​ ​only​ ​used​ ​red​ ​potatoes.​ ​We​ ​could​ ​also​ ​use​ ​different​ ​disaccharides​ ​instead​ ​of ​ ​ ​ ​ ​ ​ ​ ​ ​just​ ​sucrose. ...
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