lab report Enzyme Activity wk 1.pdf

lab report Enzyme Activity wk 1.pdf - LABORATORY REPORT...

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LABORATORY REPORT Activity: Enzyme Activity Name: Katie McClure Instructor: Dr. Bolin Date: 09.20.2017 Predictions 1. Sucrase will have the greatest activity at pH 7 2. Sucrase will have the greatest activity at 10 °C (50 °F) 3. Sucrase activity does not change with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2. Independent Variable pH 3. Controlled Variables temperature, amount of substrate (sucrose) present, sucrase + sucrose incubation time Effect of Temperature on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2. Independent Variable temperature 3. Controlled Variables pH, amount of substrate (sucrose) present, sucrase + sucrose incubation time Effect of Substrate Concentration on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2. Independent Variable amount of substrate (sucrose) present 3. Controlled Variables temperature, pH, sucrase + sucrose incubation time 4. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity. Optimal density is measured because it measure the amount of glucose and fructose produced 5. Explain why denatured sucrase was used as a control. Denatured sucrose will not have as much activity as the other sucrose Laboratory Report/ Katie McClure / Enzyme Activity/ Dr. Bolin/ 09.20.2017/ Page [1] of [4]
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6. Addition of DNS at the end of the incubation period stopped the reaction by denaturing sucrase. Explain why it is important to denature sucrase before measuring product concentration.
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