BI 205_Week_4_lab_2017.docx - Benjamin Miller\/Bi 205\/Sec 402 Week 4 Lab Osmosis and Diffusion Record the volumes of water and stock solution that you

BI 205_Week_4_lab_2017.docx - Benjamin Miller/Bi 205/Sec...

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Benjamin Miller/Bi 205/Sec 402 2/4/17 Week 4 Lab – Osmosis and Diffusion Record the volumes of water and stock solution that you used to make 200 mL of 15% sucrose below. You will need the V 1 value of the stock solution + water, so that it will add up to a total volume of 200 ml. mL of water needed = ____100________ mL of 30% stock sucrose solution needed = ____100__________ Describe your “unknown” sugar solutions here. For example, you could say, “I dissolved 1 tablespoon (~15 mL) of honey in 100 mL tap water, and poured 5 mL of this into one dialysis bag, and I poured 5 mL of room temperature orange juice into the other dialysis bag.” Two ml of maple syrup was placed in 20 ml of purified water; this was placed in the dialysis bag. Five ml of o.j. was piped into the dialysis bag. In addition, take a photograph of your experimental set-up (the dialysis bags in the beaker), and insert it here.
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7. Measure the weights of your four filled dialysis bags using the digital scale. Record your measurements below: Blue rubber band bag = __6.7 g____________ Red rubber band bag = ____6.5 g___________ Yellow rubber band bag = __6.8 g_____________ Green rubber band bag = ____6.3 g___________ White rubber band bag = _____6.9 g__________ Using the examples provided in the link above, develop a hypothesis for your experiment. Include your hypothesis, along with supporting scientific reasoning in the space below. I hypothesize that those solutions contained within the dialysis tubules which have concentrations of polysaccharides hypotonic (relative to the external solution of 15% sucrose) will experience a net loss of H 2 O. Further, those tubules which contain solutions which are hypertonic (relative to the external solution) will experience a net gain of H 2 O. Therefore, the weight of those tubules experiencing net water loss
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will be less than the original measurements indicated in the table above, whereas the weight of those tubules experiencing net water gain will be greater than the original measurement recorded in the table above.
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  • Spring '17
  • Andrew Bouwma
  • Biology

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