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Benjamin Miller/Bi 205/Sec 4022/4/17Week 4 Lab – Osmosis and Diffusion Record the volumes of water and stock solution that you used to make 200 mL of 15% sucrose below. You will need the V1value of the stock solution + water, so that itwill add up to a total volume of 200 ml.mL of water needed = ____100________mL of 30% stock sucrose solution needed = ____100__________Describe your “unknown” sugar solutions here.For example, you could say, “I dissolved 1 tablespoon (~15 mL) of honey in 100 mL tap water, and poured 5 mL of this into one dialysis bag, and I poured 5 mL of room temperature orange juice into the other dialysis bag.” Two ml of maple syrup was placed in 20 ml of purified water; this was placed in the dialysis bag.Five ml of o.j. was piped into the dialysis bag.In addition, take a photograph of your experimental set-up (the dialysis bags in the beaker), and insert it here.
7.Measure the weights of your four filled dialysis bags using the digital scale. Recordyour measurements below:Blue rubber band bag = __6.7 g____________Red rubber band bag = ____6.5 g___________Yellow rubber band bag = __6.8 g_____________Green rubber band bag = ____6.3 g___________White rubber band bag = _____6.9 g__________Using the examples provided in the link above, develop a hypothesis for your experiment. Include your hypothesis, along with supporting scientific reasoning in the space below.I hypothesize that those solutions contained within the dialysis tubules which have concentrations of polysaccharides hypotonic (relative to the external solution of 15% sucrose) will experience a net loss of H2O. Further, those tubules which containsolutions which are hypertonic (relative to the external solution) will experience a net gain of H2O. Therefore, the weight of those tubules experiencing net water loss
will be less than the original measurements indicated in the table above, whereas the weight of those tubules experiencing net water gain will be greater than the original measurement recorded in the table above.