3023-EXAM THREE Studyguide.docx

3023-EXAM THREE Studyguide.docx - MCB 3023 Study guide for...

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MCB 3023 Study guide for EXAM THREE 1. List tools used in genetic engineering and recombinant DNA technology 2. Describe, Explain, Define: PCR, RT, cDNA, Gel Electrophoresis, Vectors, Restriction enzymes, Blotting, Inserting vector into host cells a. What is the significance of primers and Tag polymerase in PCR b. What is the advantage of RT-PCR (qPCR) over traditional technique 3. What is meant by recombinant DNA? Name the tools used to make a recombinant plasmids, genomic library, or run genomic fingerprinting (RFLP) a. What is the purpose (goal) of constructing a genomic library for an organism b. Define metagenomic and its role in identifying microbes that cannot be cultured (isolated) from the environmental samples such as soil and water samples 4. What is meant by autonomous replication? 5. What does cloning mean? Compare and contrast the common cloning vectors used in creating Recombinant DNA. What is the significance of Ti plasmid? 6. What is the medical significance of comparative genomics analysis? RFLP? 7. What is meant by protoplast fusion? Electroporation? Transformation (plasmids picked up by laboratory-induced competent bacterial cells). Compare in vivo and in vitro transformation (done in lab vs. done in nature) a. What is the role of Transposons in genetic variation, mutation, and genetic exchange b. Define: Transposons, Transposition, Insertion Sequences (IS), Transposable Elements 8. Name some of the industrial and medical products produced via biotechnology 9. List some of the Human peptide and proteins synthesized by genetic engineering 10. What is meant by genetic manipulation 11. What does metabolic engineering mean? Explain DNA shuffling and whole genome shuffling. 12. How industrial microbiology defines fermentation? Compare Chemostat vs batch for large scale industrial growth 13. How reverse vaccinology works? 14. Name few categories of products made by microbes in industrial scale (industrial microbiology) 15. List the type of genes carried on plasmids. How plasmids are transferred between bacteria cells?
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