Lecture9 2017.pdf -...

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2/2/17 1 How do oxygenic photoautotrophs acquire C? !Light reac,ons: produce ATP and NADPH !Dark reac,ons : chemical energy is used to reduce CO 2 to organic compounds Energy=ATP Reducing power=NADPH ! Calvin!Benson cycle 6 CO 2 + 12 NADPH + 18 ATPJJJJ>fructoseJ6Jphospate + 12 NADP+18 ADP+ 17Pi Key enzymes: ribulose bisphosphate carboxylase oxygenase (RubisCO)
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2/2/17 2 How do anoxygenic phototrophs acquire C? (some are photoheterotrophs) Green sulfur bacteria reverse citric acid cycle 3 CO 2 + 5 ATP + 12 H (ferredoxin)JJJ> trioseJP Green nonsulfur bacteria hydroxyproprionate pathway 2 CO 2 + 3 ATP + 4 HJJJJ>glyoxylate Purple sulfur and nonsulfur bacteria CalvinJBenson cycle Nitrogen sources JNH 3 JNO 3 , NO 2 (assimilatory reducYon) JN 2 (nitrogen fixaYon) Jamino acids, purines, pyrimidines Redfield RaYo C:N:P 106:16:1
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2/2/17 3 Major N types: NO 3 J, NO 2 J, NH 4 +, N 2 gas, organic N Ammonia assimilaYon GOGAT cycle, Uses ATP, NADPH For cell use
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2/2/17 4 Assimilatory Nitrate reducYon Nitrate converted to NH4+ for amino acid biosynthesis 2 Enzymes: Nitrate reductase and Nitrite reductase (requires Fe, Molybdenum, Reductants)
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