Lecture10.Metabolism.2017.pdf - How do cells generate...

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1 How do cells generate energy (ATP)? -Photophosphorylation Light energy is absorbed and creates a charge separation across membranes to generate PMF - Fermentation Substrate-level phosphorylation. High-energy phosphate is transferred from a phosphorylated organic compound via ADP to ATP -Respiration Oxidative phosphorylation. Reduced organic or inorganic compounds are oxidized with reduction of an electron acceptor. Creates charge separation across membranes to generate PMF
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2 Fermentable substrates: Sugars, purines, pyrimidines, amino acids How do cells generate energy (ATP)? -Photophosphorylation Light energy is absorbed and creates a charge separation across membranes to generate PMF -Fermentation Substrate-level phosphorylation. High-energy phosphate is transferred from a phosphorylated organic compound via ADP to ATP - Respiration Oxidative phosphorylation. Reduced organic or inorganic compounds are oxidized with reduction of an electron acceptor. Creates charge separation across membranes to generate PMF
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3 Redox reactions Oxidation: Loss of 1 or more electrons Reduction: Gain of 1 or more electrons Reduction potential: The tendency to become oxidized (give up electrons) or to become reduced (accept electrons). Many different kinds of “respiration” Electron Donor Electron Acceptor Carbon Oxygen Ammonia Oxygen Carbon Nitrate Etc. Sulfate, etc
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4 Carbon molecules are oxidized and oxygen is reduced.
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  • Spring '08
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