Bio.Lab-Ch.3 - Bio Lab Chapter 3 Optical Microscopy 3.1...

Info icon This preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Bio. Lab Chapter 3- Optical Microscopy 1. 3.1 Structure and Function of the Light Microscope o Anything used to magnify an image is a microscope Light + lens= light microscope Must include 3 parts: Viewing medium Focusing apparatus Detection device For a long time, only a single piece of lens was used. It was called a biconvex lens . They are called simple microscopes When no actual image is projected, the image is called a virtual image . When an actual image is produced (like with an overhead) , then the image is called a real image . Modern microscopes are called compound microscopes . The basic features of the compound microscope are: Light travels from a light source through a collective of lenses called a condenser . The condenser serves to shape the direction of the light, control the intensity of the light, and the size of the beam that will hit the object. The light then travels through, reflects off of, and is absorbed by the object. When traveling from a higher refractive index to a lower refractive index, the rays will be bent to the opposite direction. When light rays pass from a lower refractive index to a higher refractive index, then they will be bent slightly perpendicular to the original path. After passing through the object, the light will either pass through air, lenses, or oil. They will travel in the direction dependent upon the medium through which they passed after traveling through the object. Then they will go through a magnifier called an objective .
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
They will then pass through to another lens called the ocular (eyepiece). Then the individual will see a virtual image. Refer to Fig. 3-5 for labeled picture of microscope. Some microscopes may have a prism within the tube that will split the light into two separate ocular lenses. This is referred to as a binocular microscope. (some may even be triocular in which the third tube is used to photograph the image. Magnification of an individual lens is: Magnification of lens= length of image produced/length of actual image magnified This is referred to 43x or any other magnification on the objective. Magnification of the whole microscope is: Total magnification= magnification of the objective x magnification of the ocular Objects can be measured if the microscope is equipped with a measuring tool called a reticle .
Image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern