Lab # 3 discussion In lab number three we made four different broth tubes. Two were synthetic and two were non-synthetic and one of each was placed in the auto clave and one of each placed in the incubator. Both the tubes placed in the incubator had growth that looked like sediment and the synthetic one that was placed in the auto clave ended up having growth. The ones in the auto clave should have had no growth at all so this could have been due to contact with something after being pulled from the auto clave. Someone may have accidently opened the lid in a non-sterile environment allowing bacteria to enter the tube. Being able to properly autoclave anything in microbiology is important for aseptic techniques and getting rid of any form of bacteria in the lab environment. For the complex mediums and the synthetic mediums almost all of them had growth except for the Enterococcus faecalis and the Staphylococcus aureus in the synthetic medium cultures. Most of the bacteria in the broth had a sediment or
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- Spring '08
- Hong-Chuan Lin