EXPERIMENT 2BEER’S LAWADDITIONAL READINGThe concepts in this experiment are discussed below. Read all the background information carefully.CONTEXTIn experiment 1 you learned how titration can be implemented to determine the concentration of acidic or basicsolutions, provided that one has an appropriate standard and end point indicator. While titration is an essential toolin your chemistry tool belt, it would not be particularly helpful in determining the concentration of a solution which isnot acidic or basic. It is also likely that, as you pursue independent research in the future, you will have so small anamount of a precious material (you will have invested a lot in it in terms of either money or time/effort) that you willnot want to compromise the material by adding impurities like indicator, etc. even if it is acidic or basic. It is atmoments like these that spectroscopy (the study of the interaction between light and matter) becomes verypowerful.In this experiment, you will be studying colored solutions using visible light spectroscopy (colorimetry) that will letyou gain a greater qualitative understanding while doing a quantitative exercise. You will learn about invisible lightspectroscopy like infrared (IR) and ultraviolet (UV) spectroscopy (these are strictly quantitative, as you can’t“eyeball” transmission of invisible radiation) in greater depth when you study organic, biological, and analyticalchemistry, but they operate on the same fundamental principles.Like titration, Beer’s Law is not just another box to be checked off on a list and cast off as old science that is nolonger relevant. Every lab doing state-of-the-art research on DNA/RNA research anywhere in the world uses Beer’slaw on a daily basis to determine concentrations of their nucleic acid solutions. It is also a stepping stone tounderstanding other interesting chemical behaviors like fluorescence and phosphorescence. OBJECTIVESAs you will be asked to do some experimental design before the end of the semester, you are encouraged to think about what each experiment is designed to probe. In addition to (re-)learning lab-related words/phrases like wavelength, frequency, quantized, photons, absorption, transmission, etc., what concept(s) does this experiment reinforce? Such reinforcement is the objective of the lab. SUMMARYThis experiment is divided into three parts to be done in one lab meeting.Part A:You will prepare a stock solution containing a sample of MiO®(a liquid water enhancer used to make fruit-tasting drinks; see ). A spectrophotometer will be used to measure the absorbancespectrum of the solution from which the wavelength at which it absorbs the maximum amount of light, max, will beobtained.