03. Sepration_2y_2016_03_16.pdf

03. Sepration_2y_2016_03_16.pdf - Countercurrent...

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Countercurrent extractions Countercurrent extraction is a liquid-liquid partition system in which no solid matrix is required to hold the stationary phase. Sequence of extractions Transfer the extracting phase to the next tube and add fresh phase to the first.
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Extraction 1 Transfer 1a Transfer 1b Extraction 2 Transfer 2 Extraction 3 2
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Example A single extraction with an organic phase removes 3% of A and 97% of B.
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Analyte with larger D will move rapidly with the mobile phase. Third extraction Second extraction
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