04. Sepration_2y_2016_03_29.pdf

04. Sepration_2y_2016_03_29.pdf - Paper Chromatography ...

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Paper Chromatography Purpose Use the technique of paper chromatography to separate a homogeneous mixture into its individual components Uses Separation Identification Water on Chromatography paper stationary phase Solvent Mobile phase
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Paper Chromatography Ascending development Descending development
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Circular development Paper Chromatography
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Mobile Phase 1 2D Paper Chromatography
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Mobile Phase 1 2D Paper Chromatography
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Mobile Phase 2 Mobile Phase 1 2D Paper Chromatography
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Mobile Phase 2 Mobile Phase 1 2D Paper Chromatography
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Type of Chromatography by separation mechanism Adsorption Chromatography solute adsorbes on to surface of solid stationary phase eg. GSC, HPLC, TLC
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Type of Chromatography by separation mechanism Partition Chromatography solute equilibrates between mobile phase and liquid stationary phase on the surface of support or column wall eg. GLC, HPLC, TLC
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Type of Chromatography by separation mechanism Ion exchange Chromatography (IEC) Anionic or cationic sites on the surface of stationary phase (resin) attract ions of opposite charge by electrostatic forces.
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