BME200_Assignment_Four.pdf - BME200 Fall 2012 Problem Set#4...

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BME200 Fall 2012- Problem Set #4 Assigned: 9/27/13 Date Due: 10/4/13 at 3 pm to be turned into the BME drop box outside of ELW A136 Please answer these questions on a separate sheet of paper along with your name, assignment number, and student number. 1. In general terms, how do enzymes speed up biological reactions? 2. For an enzyme (5 μM) , the following initial velocities have been reported depending on the substrate concentration: (a) Draw a Michaelis-Menten plot for this enzyme. (b) Draw a Lineweaver-Burke plot for this enzyme. (c) Determine K M and Jmax for this enzyme. (d) Indicate in both graphs (a & b) where Jmax and K M can be determined. 3. Why are the diffusion coefficients slower in the cytoplasm of cells than in water? 4. The intestinal enterocytes form a covering over the intestinal lining which, to the first approximation, can be considered to be a plane. Assuming no binding or sequestration within the cell, what is the estimated time of diffusion of Ca
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  • Fall '16
  • Rustom Bhiladvala

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