Journal Review 3 .docx - Minh Doan Water Permeation Drives Tumor Cell Migration in Confined Microenvironments Cell Kimberly M Stroka Hongyuan Jian

Journal Review 3 .docx - Minh Doan Water Permeation Drives...

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Minh Doan Water Permeation Drives Tumor Cell Migration in Confined Microenvironments Cell Kimberly M Stroka, Hongyuan Jian, Shih-Hsun Chen, Ziqui Tong, Denis Wirtz, Sean X. Sun, and Konstantinos Konstantopoulos Dr. Stroka’s research is focused upon cell and tissue engineering. Specifically, she studies how external biochemical and mechanical forces may affect cell activity and disrupt cell homeostasis. Her main purpose is to use these studies to develop therapeutic treatments for diseases and cancer. In this article, she focuses on the osmotic engine model, which claims that the cell can use aquaporins and ion channels to regulate osmolarity, which leads to cell movement. One of the techniques used in this lab was the utilization of microfluidic device to measure cell migration through its micropores by measuring the velocity through each of the pores. As the cells migrate through the pores, sensors are used to measure the speed of flow. The pore size was manipulated to observe how these may affect the speed of migration and find the optimum speed. Another technique used in this lab was known as osmotic shock, which changes the amount of ions inside and outside the cell, leading to changes in osmotic pressure. Osmotic shock was used to test the dependency of osmotic pressure on cell migration speed as well as the direction in which the cell migrated. This was done by manipulating the osmolarity of the cell by
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  • Spring '14
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