Discussion Section Week 5 - Questions.pdf - Discussion Section Practice Problems Source UC Berkeley Chemical Engineering CHE170A 1 Consider the growth

Discussion Section Week 5 - Questions.pdf - Discussion...

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Discussion Section Practice Problems October 30, 2017 Source: UC Berkeley Chemical Engineering CHE170A 1Consider the growth of a micro-ogranism in batch culture. When the substrate concentration is high,the cell density doubles every 0.75 hours, the observed substrate yield coefficient is 0.3 g DCW (dry cellweight)/g and substrate consumption is allocated towards biosynthesis (60%), maintenance (10%), andproduct formation (30%). The product formation is strictly growth-associated.The batch reactor is inoculated with 0.01 g DCW/L and 10 g/L substrate.1aEstimate the maximum cell density and the time (after lag phase) required to achieve it.1bDetermine the value of the maintenance coefficient (g substrate / g DCH -h).1cBefore inoculation of the batch reactor, you need to sterilize the medium, which contains 105sporesL. Thevalue ofkdhas been determined to be 1min-1at 121Cand 61min-1at 140C. For each temperature,
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