44-0306-00-02 RPT Bacterial Enumeration-Dilutions and Plate Counts (Daisy Calderon ).docx

44-0306-00-02 RPT Bacterial Enumeration-Dilutions and Plate Counts (Daisy Calderon ).docx

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Daisy Calderon Student ID: 2178674 Kit Code: 2016-LP00037631 Bacterial Enumeration-Dilutions and Plate Counts Hands-On Labs, Inc. Version 42-0306-00-02 Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor. Exercise 1: Dilutions and Viable Plate Counts Data Table 1. Dilution Plate Counts Dilutio n Photo Count and Viability (Viable, TFTC, or TNTC) 10 -1 33; Viable
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Daisy Calderon Student ID: 2178674 Kit Code: 2016-LP00037631 10 -2 13 TFTC 10 -3 10 TFTC 10 -4 6 TFTC
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Daisy Calderon Student ID: 2178674 Kit Code: 2016-LP00037631 10 -5 2 TFTC 10 -6 1 TFTC Table 2. Calculations of Original Sample CFU/mL. Calculation Answer Dilution Plate CFUs 33 Dilution Factor of Viable Plate 1:10 Volume of Diluent added to Plate 0.1ml CFU/mL of Original Sample: CFU/(Volume x Dilution Factor) 3,300 CFU/ml
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