Cell Structure Lecture.pdf - Microbiology 303 Cell structure and function Outline I General Thoughts about Cell Structure II Visualizing Microorganisms

Cell Structure Lecture.pdf - Microbiology 303 Cell...

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Microbiology 303Cell structure and function
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OutlineI. General Thoughts about Cell Structure II. Visualizing Microorganisms III. The Cytoplasm A. Nucleoid B. Ribosomes C. Cytoskeleton D. Inclusions IV. The Membrane A. Membrane Lipids B. Transport Across the membrane V. Outer Layers A. Cell envelopes i. Peptiodglycan ii. Gram-positive iii. Gram-Negative B. Cell wall diversity C. Capsules D. Pili E. Flagella VI. Archaea A. Cell Membranes B. Cell Wall Types VII. Eukaryotes A. Organelles B. Origin of Eukaryotes
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Learning outcomesBe able to recognize the structures of the monomersthat make up the basic building blocks of the cellBe able to assign a function to a structural unit.Cytoplasm, nucleoid, membrane.Be able to recognize DNA and protein structuresDecide on the correct type of microscopy andsample preparation for a given situation.Explain how bright-field microscopy works and whyspecimens must be stained.
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Learning outcomesList two structures that both Gram-negative and Gram-positive cellshave in common, and provide the function of each.List two structures that are unique to Gram-negative and to Gram-positive cells, and provide the function of each.Distinguish between cell envelope structures (e.g., membranes andcell wall, etc.) in Gram- positive and Gram-negative bacteria.Compare and contrast the different cellular transport processes(e.g., facilitated diffusion, ion driven transport/simple transport,ABC transporter, group translocation, etc.) with regard to theproteins involved and the energy source used.Distinguish between different modes of motilityExplain what chemotaxis is and how it worksCompare and contrast the structure of cell membranes and cellwalls in Bacteria, Archaea, and Eukarya
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For microbes: the cell is the organism and Cells are CellsThe basic unit of lifeAll cells are made of basically the same things:proteins, nucleic acids, lipids, sugars, andvarious small moleculesMembranes are the critical divider betweenself and non-selfMaintain barrierSense outside worldContain cell components
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Microbes have the same basic life functions asmacrobes(as populations)Common to all microbes-3, 4 and 5 many but not all microbes
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On being small: surface to volume ratio
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Cell shape variesBacillus (rod)Spirochete
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Why so many different shapes?Nutrient acquisitionDefense from predatorsPhagocytes that eat cells, multicellular microbivoresAttachment to surfacesDispersalMotilityDifferentiationWhy not?
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Visualized Structures UsingLight microscopeFluorescent microscopePhase-contrast microscopeConfocal microscopeAtomic force microscopeElectron microscope (SEM, TEM)X-ray CrystallographyJanice Carr/ Jeff Hageman, M.H.S.CDC
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Visualizing microbes 1. Your brilliant, but absent-minded, research mentor is terribly excited.
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