enzymeprocedures

enzymeprocedures - Enzymes Lab Procedures INITIAL PROCEDURE...

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Enzymes Lab Procedures INITIAL PROCEDURE 1) Label the cuvettes 1 through 4 in a place that does not obstruct the reading in the spectrophotometer. 2) Add the contents (indicated by the chart below into the correct cuvette, always adding the enzyme solution last. Catechol Soln Dist. H 2 O Enzyme Soln Cuvette 1 2 mL 3 mL 0 mL Cuvette 2 0 mL 4 mL 1 mL Cuvette 3 2 mL 2 mL 1 mL Cuvette 4 (Calibr) 0 mL 5 mL 0 mL 3) Carefully invert the cuvette after covering with a piece of parafilm. 4) Wipe the exterior of the cuvette with a Kimwipe and place cuvette 4 into the spectrophotometer. 5) Move the zero knob on the spectrophotometer until it reads 0.0 absorbency. Remove cuvette 4. Immediately after measuring the initial absorbency, place the cuvettes into a 37°C water bath. 6) Place cuvette 1 into the compartment after wiping with Kimwipe and inverting. Without moving the knob, record the absorbency as displayed. 7)
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enzymeprocedures - Enzymes Lab Procedures INITIAL PROCEDURE...

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