Formal Lab Report 2 - Beers Law and the Concentration of...

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Beer’s Law and the Concentration of Color Aaron Gliner Lab Partner: Rachel Brown Date of Experiment, March, 2, 2016 Abstract: Spectroscopy is the study of the interaction of matter and radiated energy. Electrons move through orbitals by emitting a photon of light. Beer’s law states that the more molecules there are to absorb photons, the higher that substance’s absorbance will be. Using Beer’s Law (A=Elc), the Absorbance of a substance can be found by first finding its concentration which is directly proportional to the absorbance. In this experiment, we found the absorbance and concentration of the commercial product and its correlation to other standard solution’s concentration and absorbance. To do this, the wavelength of maximum absorbance (Lambda Max) of the mouthwash was found first by plotting absorbance vs wavelength (Graph 1). With the given concentration of the mouthwash at 7.75E-6 M and the lambda max at 624.4 nm, the other standards’ concentrations can be found. The 3 standards contain one with 5mL of stock solution and 1mL of DI water, one with 1mL of stock solution and 5mL of DI water, and one with 3mL of stock solution and 3mL of DI water. Once the concentration of those were found, each individual

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