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cultureintroduction.pptx - Animal Microtechniques Tissue...

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Animal Microtechniques & Tissue Culture Introduction to Cell Culture
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What Does It Mean To “Culture” Cells? Isolated cells Provision of appropriate environment Gas Substrate Medium Incubator: 5% CO 2 , 37° C, humidified
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Why Culture Cells? To study biological phenomena Gene transcription Cellular metabolism Transport activities Cell-cell interactions Mechanism of drug action High throughput candidate screening Pharmaceutical production
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Advantages vs. Disadvantages Advantages Control of environment Homogeneous, well- characterized sample Economical Disadvantages Expertise Quantity of cells Potential instability of cells in culture Availability of appropriate models
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Brief History of Tissue/Cell Culture Whole animal studies From earliest time of biological observations Response to an applied treatment “Feed ‘em & Weigh ‘em” Clinical drug trials
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Brief History of Tissue/Cell Culture
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