cultureenvironmentC.ppt - The Culture Environment BSL4...

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The Culture Environment
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BSL4
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Components of the Culture Environment The Gas Phase The Substrate Growth surface The Medium / Media
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The Gas Phase Short-term cultures 95% O 2 : 5% CO 2 Long-term cultures Air + CO 2 to 5% Add sodium bicarbonate Buffer in media Cellular requirement for bicarbonate Balanced salt solutions Air - Hank’s balanced salts: 0.35 g / l 5% CO 2 - Earles’s balanced salts: 2.2 g / l
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The Substrate Glass Plastic (usually polystyrene) Pretreated to make a hydrophilic surface Net + charge Many types
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Culture Vessels – Descriptions and Surface Areas
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T-Flasks
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T-Flask Permeable Caps
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Petri Dishes
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Vented Dish
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Multiwell Plates
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Chamber Slides
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Roller Bottles
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Roller Bottle Cross-Section
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Nunclon “Cell Factory”
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Filling the Nunclon “Cell Factory”
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The Substrate Glass Plastic (usually polystyrene) Pretreated to make a hydrophilic surface Net + charge Many types Microcarriers 100 m diameter Increase cell density / unit volume of media Useful for bioreactors
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Microcarrier Beads with Attached Cells
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Stirrer Flasks for Microcarrier Beads
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  • Spring '15
  • jesse

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