UNIT 1-Lab Notes 2.Microscopy for Microbiology.pdf - UNIT...

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UNIT 1-Lab Notes: 2. Microscopy for Microbiology Microscope→ optical instrument that uses a lens or a series of lenses to magnify objects that are either too small to be seen by the naked eye, or to view distinct details of an object that are not visible to the naked eye. The categories are: optical, electron and scanning microscopes. Optical microscopes aka light microscopes , use reflected visible light and a series of two or more convex lenses to magnify and focus an object. Compound microscope→ the most common type of light which utilizes a lens of short focal length and a second lens of a longer focal length. the short lens forms and focuses the image, the longer lens magnifies and further focuses the image. they are able to resolve objects to a resolution of approximately 200 nm (0.2 μm). Resolving power → the ability to discern two objects as separate, and is limited by the wavelength of light Microscopes Components: ocular lens→ short lens located at the top of the microscope. It is often referred to as the eye piece and is typically 10x or 15x power.

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