BIOL 202 Final Exam study guide

BIOL 202 Final Exam study guide - BIOL 202 Spring 2007...

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BIOL 202: Spring 2007 Final Exam review: Chapter 8 I. The Source of the Donor DNA o donor organism- t he organism which will be used to donate DNA for  analysis is called . o Any organism’s DNA can be purified in a test tube by straightforward  isolation procedures: Cells of donor are broken apart and DNA is separated  o Three Sources of DNAsegments: Genomic DNA     Exists in the chromosomes of the organism… straightforward source of DNA Complentary DNA (cDNA)     Essentially a doubled stranded DNA version of an mRNA  molecule. Made from mRNA with  reverse transcriptase Reverse transcriptase synthesizes a single stranded DNA  molecule that can then be used as a template for double  stranded DNAsynthesis Chemically Synthesized DNA     When a recombinant DNA molecule (a specific sequence  that cannot be isolated from available natural genomic or  cDNAs) Highly automated techniques for the synthesis of  oligonucleotides (12-100nucleotides) have been developed  for this purpose Chopping Up the Donor DNA DNA can be broken by  physical shearing (agitating it in a blender)
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enzymatically digesting it with endonucleases that cleave at specific sites
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This note was uploaded on 03/20/2008 for the course BIOL 202 taught by Professor Kieber-hogan during the Spring '08 term at UNC.

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BIOL 202 Final Exam study guide - BIOL 202 Spring 2007...

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