LAB 1 REVIEW - LAB 1 REVIEW Chapter 1 Info Laboratory...

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Unformatted text preview: LAB 1 REVIEW Chapter 1 Info: Laboratory Practice and Safety • Genetic Engineering: information content of a living organism is permanently changed using techniques of molecular biology • Biotechnology: broad range of enterprises, often using genetically engineered organisms • Field scientists: study phenomena of nature in their normal setting • Laboratory scientists: study material in a laboratory • Biomolecules: molecules synthesized by living cells; often produced in small amounts; difficult to separate • Wet Laboratory : room equipped with lots of running water and drains, high benches, floors that are easily mopped, surfaces that are relatively inert to chemical agents, and cabinets for storing glassware and chemical substances; include a ventilated work area (fume hood); refrigerators and freezers • Instrument laboratory : equipped with low benches or tables; devoid of stored toxic chemicals; expensive electronic instruments, computing equipment and reading areas Units of Measure and Measuring Devices • Weight: top-loading balance (2-place accuracy) is used to weigh specified amounts of substances quickly; analytical balance (4-place accuracy) is specially designed to accurately weigh very small quantities; two-pan balance is used to compare the weights of two items (used prior to subjecting vessels to centrifugation) • Calibrated beakers and Erlenmeyer flasks: 5% accuracy with volumes of over 50% • Graduated cylinders and pipets: 1% accuracy when over 50% of their capacity o 5mL to 2 L • Pipet: transparent tube with a narrow tip o 1 and 10mL • Delivery Pipet: o Major 1-mL graduations, smaller 1/10 mL graduations (10mL total) • Blow-Out Pipet: o No 10-mL graduation line; 10mL total; not accurate between 9.5-10mL • Pipet Pumps: Use a piston system to create the vacuum and draw liquid into a pipet and allow controlled volumes to flow out again • Automatic Pipetters: for 1µ to 1mL o Variable-volume automatic pipette can be adjusted to deliver any volume within a specified range; WHAT WE USE ; locking mechanism; liquid is pushed out of the tip by releasing a vacuum and providing a slight positive pressure through the tip Laboratory preparation of Aqueous Solutions •...
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This note was uploaded on 03/20/2008 for the course BIO 205L taught by Professor Hanson during the Fall '07 term at University of Texas.

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LAB 1 REVIEW - LAB 1 REVIEW Chapter 1 Info Laboratory...

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